Researcher: Dr. Ginés Viscor
Exploratory study to determine the potential efficacy of intermittent exposure to hypoxia in hypobaric chamber in recovery from muscle damage induced in rats.
Muscle damage is induced in the hindquartes of the laboratory animals through a session of forced downhill running in accordance with protocols that are widely validated and accepted by the scientific community.
Our primary goal is to describe the evolution of plasma markers of muscle damage during recovery in control rats and treated rats. Meanwhile, we are developing a more detailed study of the structural and biochemical changes induced in the muscles of the rat's hind legs.
The animals are divided into three groups: one control group and two experimental groups. the control animals [AMB] go through a passive recovery, living a normal, inactive life.
All the experimental animals, however, are administered a programme of intermittent exposure to hypobaric hypoxia. Half the experimental animals [HIP] are given no additional treatment, while the othe half [EHIP], in addition to being exposed to simulated high altitude, are subject to an exercise protocol that is equivalent to a programme to maintain physical fitness /scaled down to the size of the rats).
At each sampling phase some animals (n = 7) are euthanised so the mechanisms involved in the potential improved recovery can be analysed in greater detail by examining samples of muscle and other tissues.
For this reason histochemical, immunocytochemical and transcriptomic studies are conducted to assess both structural damage and subsequent changes to that damage.
NB: This study is co-funded by the National Research, Development and Innovation Programme (DEP2010-22205-C02-01).